Molecule: S. avermitilis chromosome S. avermitilis plasmid SAP1 S. avermitilis plasmid SAP2 S. avermitilis SUKA22 S. avermitilis SUKA23 S. coelicolor A3(2) chromosome S. coelicolor A3(2) plasmid SCP1 S. coelicolor A3(2) plasmid SCP2 S. griseus IFO13350 chromosome S. ambofaciens ATCC 23877 chromosome S. lividans TK24 chromosome S. lividans plasmid SLP2 S. albus J1074 chromosome S. scabies 87.22 chromosome S. bingchenggensis BCW-1 chromosome S. clavuligerus ATCC 27064 chromosome S. clavuligerus ATCC 27064 plasmid pSCL4 S. cattleya NRRL 8057 chromosome S. cattleya NRRL 8057 plasmid pSCAT S. venezuelae ATCC 10712 chromosome S. parvulus ATCC 2297 chromosome S. parvulus plasmid pSPA1 S. viridochromogenes DSM 40736 chromosome S. cacaoi subsp. asoensis JCM 4185 chromosome S. kanamycetis ATCC 12853 chromosome S. lincolnensis NRRL 2936 chromosome S. fradiae ATCC 10745 chromosome S. violaceusniger Tu 4113 chromosome Streptomyces sp. SirexAA-E chromosome S. flavogriseus ATCC 33331 chromosome S. hygroscopicus subsp. jinggangensis 5008 chromosome S. davawensis JCM 4913 chromosome S. davawensis JCM 4913 plasmid pSDA1 S. fulvissimus DSM 40593 chromosome S. fulvissimus DSM 40593 plasmid pSFLA01 S. fulvissimus DSM 40593 plasmid pSFLA02 S. collinus Tu 365 chromosome S. rapamycinicus NRRL 5491 chromosome Streptomyces. sp. PAMC26508 chromosome S. mooreae ATCC 21705 chromosome Kitasatospora setae KM-6054 chromosome Saccharopolyspora erythraea NRRL2338 chromosome Verrucosispora_maris AB-18-032 chromosome Amycolatopsis mediterranei U32 chromosome Actinoplanes missouriensis 431 chromosome Nocardioides sp. JS614 chromosome Nakamurella multipartita DSM44233 chromosome Saccharomonospora viridis DSM43017 chromosome Actinosynnema mirum DSM43827 chromosome S. violaceoruber plasmid pSV2 S. rochei plasmid pSLA2-L Bacteriophage phiC31 Bacteriophage phiBT1 Bacteriophage TG1 From 0 1 2 3 4 5 6 7 8 9 . 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 Mbp Sequence from: to: Span: 1,000 bp 2,500 bp 5,000 bp 10,000 bp 25,000 bp 50,000 bp 75,000 bp 100,000 bp 200,000 bp 250,000 bp 500,000 bp 1,000,000 bp Enzyme 1: AatII(GACGTC) AarI(CACCTGCnnnnnnnn) Acc36I(ACCTGCnnnnnnnn) AccII(CGCG) AflII(CTTAAG) AgeI(ACCGGT) AluI(AGCT) ApaI(GGGCCC) ApaLI(GTGCAC) ApoI(rAATTy) AscI(GGCGCGCC) AseI(ATTAAT) AvrII(CCTAGG) BamHI(GGATCC) BbsI(GAAGACnnnnnn) BbvCI(CCTCAGG) BciVI(GTATCCnnnnnn) BclI(TGATCA) BfaI(CTAG) BfiI(ACTGGGnnnnn) BglII(AGATCT) BmgBI(CACGTC) BseYI(CCCAGC) BsiWI(CGTACG) BspEI(TCCGGA) BspHI(TCATGA) BspLU11I(ACATGT) BsrBI(CCGCTC) BsrGI(TGTACA) BssHII(GCGCGC) BssSI(CACGAG) Bst1107I(GTATAC) BstBI(TTCGAA) BstYI(rGATCy) ClaI(ATCGAT) DraI(TTTAAA) EagI(CGGCCG) Eco47III(AGCGCT) EcoRI(GAATTC) EcoRV(GATATC) FseI(GGCCGGCC) FspI(TGCGCA) HaeIII(GGCC) HhaI(GCGC) HindIII(AAGCTT) HpaI(GTTAAC) HpaII(CCGG) IPpoI(CTCTCTTAAGGTAGC) KpnI(GGTACC) MboI(GATC) MfeI(CAATTG) MluI(ACGCGT) MscI(TGGCCA) MseI(TTAA) NarI(GGCGCC) NcoI(CCATGG) NdeI(CATATG) NgoMI(GCCGGC) NheI(GCTAGC) NlaIII(CATG) NotI(GCGGCCGC) NruI(TCGCGA) NsiI(ATGCAT) PacI(TTAATTAA) PmeI(GTTTAAAC) PmlI(CACGTG) PsiI(TTATAA) Psp1406I(AACGTT) PstI(CTGCAG) PvuI(CGATCG) PvuII(CAGCTG) RsaI(GTAC) SacI(GAGCTC) SacII(CCGCGG) SalI(GTCGAC) SapI(GCTCTTCnnnn) ScaI(AGTACT) SgfI(GCGATCGC) SmaI(CCCGGG) SnaBI(TACGTA) SpeI(ACTAGT) SphI(GCATGC) SrfI(GCCCGGGC) Sse8387I(CCTGCAGG) SspI(AATATT) StuI(AGGCCT) SwaI(ATTTAAAT) TaqI(TCGA) Tsp509I(AATT) TthHB8I(TCGA) XbaI(TCTAGA) XhoI(CTCGAG) ZraI(GACGTC) CTAG-family GATC-family Enzyme 2: nothing AatII(GACGTC) AarI(CACCTGCnnnnnnnn) Acc36I(ACCTGCnnnnnnnn) AccII(CGCG) AflII(CTTAAG) AgeI(ACCGGT) AluI(AGCT) ApaI(GGGCCC) ApaLI(GTGCAC) ApoI(rAATTy) AscI(GGCGCGCC) AseI(ATTAAT) AvrII(CCTAGG) BamHI(GGATCC) BbsI(GAAGACnnnnnn) BbvCI(CCTCAGG) BciVI(GTATCCnnnnnn) BclI(TGATCA) BfaI(CTAG) BfiI(ACTGGGnnnnn) BglII(AGATCT) BmgBI(CACGTC) BseYI(CCCAGC) BsiWI(CGTACG) BspEI(TCCGGA) BspHI(TCATGA) BspLU11I(ACATGT) BsrBI(CCGCTC) BsrGI(TGTACA) BssHII(GCGCGC) BssSI(CACGAG) Bst1107I(GTATAC) BstBI(TTCGAA) BstYI(rGATCy) ClaI(ATCGAT) DraI(TTTAAA) EagI(CGGCCG) Eco47III(AGCGCT) EcoRI(GAATTC) EcoRV(GATATC) FseI(GGCCGGCC) FspI(TGCGCA) HaeIII(GGCC) HhaI(GCGC) HindIII(AAGCTT) HpaI(GTTAAC) HpaII(CCGG) IPpoI(CTCTCTTAAGGTAGC) KpnI(GGTACC) MboI(GATC) MfeI(CAATTG) MluI(ACGCGT) MscI(TGGCCA) MseI(TTAA) NarI(GGCGCC) NcoI(CCATGG) NdeI(CATATG) NgoMI(GCCGGC) NheI(GCTAGC) NlaIII(CATG) NotI(GCGGCCGC) NruI(TCGCGA) NsiI(ATGCAT) PacI(TTAATTAA) PmeI(GTTTAAAC) PmlI(CACGTG) PsiI(TTATAA) Psp1406I(AACGTT) PstI(CTGCAG) PvuI(CGATCG) PvuII(CAGCTG) RsaI(GTAC) SacI(GAGCTC) SacII(CCGCGG) SalI(GTCGAC) SapI(GCTCTTCnnnn) ScaI(AGTACT) SgfI(GCGATCGC) SmaI(CCCGGG) SnaBI(TACGTA) SpeI(ACTAGT) SphI(GCATGC) SrfI(GCCCGGGC) Sse8387I(CCTGCAGG) SspI(AATATT) StuI(AGGCCT) SwaI(ATTTAAAT) TaqI(TCGA) Tsp509I(AATT) TthHB8I(TCGA) XbaI(TCTAGA) XhoI(CTCGAG) ZraI(GACGTC) Enzyme 3: nothing AatII(GACGTC) AarI(CACCTGCnnnnnnnn) Acc36I(ACCTGCnnnnnnnn) AccII(CGCG) AflII(CTTAAG) AgeI(ACCGGT) AluI(AGCT) ApaI(GGGCCC) ApaLI(GTGCAC) ApoI(rAATTy) AscI(GGCGCGCC) AseI(ATTAAT) AvrII(CCTAGG) BamHI(GGATCC) BbsI(GAAGACnnnnnn) BbvCI(CCTCAGG) BciVI(GTATCCnnnnnn) BclI(TGATCA) BfaI(CTAG) BfiI(ACTGGGnnnnn) BglII(AGATCT) BmgBI(CACGTC) BseYI(CCCAGC) BsiWI(CGTACG) BspEI(TCCGGA) BspHI(TCATGA) BspLU11I(ACATGT) BsrBI(CCGCTC) BsrGI(TGTACA) BssHII(GCGCGC) BssSI(CACGAG) Bst1107I(GTATAC) BstBI(TTCGAA) BstYI(rGATCy) ClaI(ATCGAT) DraI(TTTAAA) EagI(CGGCCG) Eco47III(AGCGCT) EcoRI(GAATTC) EcoRV(GATATC) FseI(GGCCGGCC) FspI(TGCGCA) HaeIII(GGCC) HhaI(GCGC) HindIII(AAGCTT) HpaI(GTTAAC) HpaII(CCGG) IPpoI(CTCTCTTAAGGTAGC) KpnI(GGTACC) MboI(GATC) MfeI(CAATTG) MluI(ACGCGT) MscI(TGGCCA) MseI(TTAA) NarI(GGCGCC) NcoI(CCATGG) NdeI(CATATG) NgoMI(GCCGGC) NheI(GCTAGC) NlaIII(CATG) NotI(GCGGCCGC) NruI(TCGCGA) NsiI(ATGCAT) PacI(TTAATTAA) PmeI(GTTTAAAC) PmlI(CACGTG) PsiI(TTATAA) Psp1406I(AACGTT) PstI(CTGCAG) PvuI(CGATCG) PvuII(CAGCTG) RsaI(GTAC) SacI(GAGCTC) SacII(CCGCGG) SalI(GTCGAC) SapI(GCTCTTCnnnn) ScaI(AGTACT) SgfI(GCGATCGC) SmaI(CCCGGG) SnaBI(TACGTA) SpeI(ACTAGT) SphI(GCATGC) SrfI(GCCCGGGC) Sse8387I(CCTGCAGG) SspI(AATATT) StuI(AGGCCT) SwaI(ATTTAAAT) TaqI(TCGA) Tsp509I(AATT) TthHB8I(TCGA) XbaI(TCTAGA) XhoI(CTCGAG) ZraI(GACGTC)
